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Full Project – THE RESPONSE OF LIPID PROFILE TO THE ADMINISTRATION OF DIOCLEAREFLEXA HOOK. F (AGBANRIN) ETHANOIC EXTRACT IN ADULT WISTAR RATS

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ABSTRACT

This research work was carried out to determine the effect of Diocleareflexa hook.F on fasting blood sugar level of adult wistar rats. Twenty wistar rats (both male and female) weighing between 140g to 160g were used for this research project. They were acclimatized for 14 days after which the animals were grouped into four groups of five rats each. Each animal were administered based on their body weight. The dose given to animals in group A was 300mg/kg body weight of Diocleareflexa hook.F, Group B was given 500mg/kg body weight of Diocleareflexa hook.F, Group C was given 700mg/kg body weight of Diocleareflexa hook.F and group D (control group) was given distilled water. The administration lasted for thirteen days. Blood samples were collected from the tail of the animals and the test for blood lipid profile level was conducted.

TABLE OF CONTENTS

Abstract

CHAPTER ONE

1.0 Introduction

1.1 Medicinal Plants

1.2 Aim of Study

CHAPTER TWO

2.0 Literature Review

2.1 Application of medicinal plants

2.1.1 Diocleareflexa Hook. F

2.2 Lipid Profile

2.2.1 Impacts of medicinal plants on lipid profile

CHAPTER THREE

3.0 Materials and Methods

3.1 Apparatus and Equipments

3.2 Chemical Reagents

3.3 Collection and extraction of plant materials

3.3.1 Diocleareflexa Hook. F

3.4 Practical history and animal management

3.5 Drug administration

3.6 Sacrificing of rats

3.7 Biochemical analysis

3.7.1 Triglycerides

3.7.1.1 Principle

3.7.1.2 Reagent composition

3.7.1.3 Preparation of reagents

3.7.1.4 Procedure

3.7.2 HDL Cholesterol

3.7.2.1 Principle

3.7.2.2 Preparation of reagents

3.7.2.3 Procedure

3.8 Statistical analysis

CHAPTER FOUR

4.0 Results

CHAPTER FIVE

5.0 Discussion, Conclusion and Recommendation

5.1 Discussion

5.2 Conclusion

5.3 Recommendations

References

Appendix

LIST OF FIGURES

Figure1 – Diocleareflexa Hook. F

Figure2 – Bar chart showing the level of cholesterol in adult wistar rats

Figure3 – Bar chart showing the level of HDL in adult wistar rats

Figure4 – Bar chart showing the level of LDL in adult wistar rats

Figure5 – Bar chart showing the level of triglycerides in adult wistar rats

LIST OF TABLE

Table 1 – Group and treatment administered

Table 2- Procedure for triglyceride determination

Table 3- Procedure for HDL determination

Table 4- Cholesterol CHOD-PAP assay

Table 5- Showing the level of cholesterol, HDL, LDL and triglycerides in adult wistar rats.

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